| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Wang Shi1, Mo Fan1, Jin Yinji2, Tian Jing1, Yao Ting3, Zhao Xinxue1, Zou Yufei4, Xiao Zhiming1* |
| 作者单位 | 1. Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. Beijing General Station of Animal Husbandry, Beijing 100107, China; 3. Beijing Veterinary Drug and Feed Monitoring Center, Beijing 102200, China; 4. Suzhou Beaver Biomedical Engineering Co., Ltd., Suzhou 215123, China |
| DOI | 10.19578/j.cnki.ahfs.2025.01-06.005 |
| 年份 | 2025 |
| 刊期 | 1 |
| 页码 | 29-34 |
| 关键词 | Immunomagnetic beads (IMBs) clean-up; High-performance liquid chromatography; Feed; Deoxynivalenol (DON) |
| 摘要 | [Objective] The aim of this work was to establish an analytical method for the determination of deoxynivalenol (DON) in feeds using automatic immunomagnetic beads (IMBs) clean-up coupled with high-performance liquid chromatography. [Method] Feed samples were extracted using ultra-pure water, purified by automatic IMBs, and subsequently analyzed via high-performance liquid chromatography, employing an external standard method for quantification. [Result] A satisfactory linearity was achieved for DON within the concentration range of 0.05 to 2.0 μg/mL, with the corresponding correlation coefficients (R2) exceeding 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) for the proposed method were determined to be 0.03 and 0.1 mg/kg, respectively. The average recoveries of the fortified samples (0.1, 0.2 and 1.0 mg/kg) were 88.5%-100.6%, with the relative standard deviations (RSD) ranging from 2.1% to 9.7%. [Conclusion] In comparison with the traditional solid-phase extraction and immunoaffinity column purification methods, the IMBs technique consolidates the extraction, separation, and purification into a single process. This approach enables fully automated processing, which significantly enhances work efficiency and mitigates result deviations that may arise from manual operations. Consequently, this technique is particularly well-suited for the determination of DON in a large number of feed samples. |
