| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Bao Tuya1, Bai Sarina1, Wu Ling1, Guo Xiaozhen1, Hou Yunpeng2, Nashun Bayaer1* |
| 作者单位 | 1. School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, China; 2. The First Clinical Medical College of Inner Mongolia Medical University, Hohhot 010000, China |
| DOI | 10.19578/j.cnki.ahfs.2025.01-06.001 |
| 年份 | 2025 |
| 刊期 | 1 |
| 页码 | 1-5 |
| 关键词 | Estradiol; Progesterone; LPS; Salpingitis; IL-1β |
| 摘要 | [Objective] The paper was to study the effects of estradiol (E2) and progesterone (P4) on the expression of interleukin-1β (IL-1β) mRNA in lipopolysaccharide (LPS)-induced acute salpingitis in ovariectomized mice, and preliminarily explore the anti-inflammatory mechanism associated with estrogen and progesterone. [Method] Healthy female KM mice were randomly assigned to several groups: the sham operation group (LPS+SHAM), the ovarian removal group (LPS+OVX), the ovarian removal + estradiol group (LPS+OVX+E2), the ovarian removal + progesterone group (LPS +OVX+P4), the LPS group and the control group (control). HE staining was conducted to assess the pathological changes in the fallopian tubes of each group. Additionally, the expression levels of IL-1β mRNA in the fallopian tubes of the mice were quantified using RT-qPCR. [Result] The histopathological changes in the fallopian tubes were examined. Estrogen and progesterone demonstrated a significant capacity to mitigate salpingitis induced by LPS. In comparison to the control group, the expression of IL-1β mRNA in the LPS group, LPS+SHAM group, and LPS+OVX+E2 group was significantly down-regulated (P<0.05). Furthermore, the expression of IL-1β mRNA in the LPS+OVX+P4 group exhibited an extremely significant down-regulation (P<0.01). When compared to the LPS+OVX group, the expression of IL-1β mRNA in the LPS+OVX+E2 group was significantly down-regulated (P<0.05), while the expression in the LPS+OVX+P4 group was extremely significantly down-regulated (P<0.01). [Conclusion] Estrogen and progesterone have the capacity to inhibit the expression of IL-1β mRNA in the inflammatory tissue of the fallopian tubes in mice, consequently diminishing the inflammatory response induced by LPS. |
